Antioxidant (DPPH) Study: Eye Refresh

1.0 OBJECTIVE: 

The purpose of this study was to evaluate the antioxidant potential of the Aeonia Eye Refresh. To measure this effect, the scavenging effect of the serum on the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) was determined. The free radical DPPH has a strong absorbance at 490 nm, and this absorbance is reduced when DPPH reacts with antioxidant compounds and is converted to hydrazine. The DPPH assay is considered a valid and easy assay to evaluate scavenging activity of antioxidants, since the radical compound is stable and does not have to be generated as in other radical scavenging assays.

2.0 Antioxidant Potential of the Serum as Determined by the DPPH Assay

The results for the DPPH assay are presented in Table/Graph 1 and are expressed as percent DPPH scavenged ± standard deviation.

Table 1: DPPH Assay Results

EC50: 0.51%

Graph 1: DPPH Assay Results

The purpose of this study was to evaluate the antioxidant capabilities of the Aeonia Eye Refresh using the DPPH assay. In this study, the Eye Refresh was observed to have antioxidant activity, with an EC50 (concentration at which it scavenged 50% of the DPPH) of 0.08%. This level of activity is equivalent to having 17.70 mg of Trolox per ml of the serum and can be expressed as 1770.34 mg Trolox Equivalents per 100g of material or 7,073 umol Trolox Equivalents per 100g of material. For reference, some standard Trolox Equivalents can be found in Table 2.

Table 2: Trolox Equivalents

Miller, H.E., Rigelhof, F., Marquart, L.,Prakash, A., Kanter, M.  Antioxidant content of whole grain breakfast cereals, fruits and vegetables.  Journal of the American College of Nutrition.  19(3): 312S-319S, 2000.

Disclaimer: This document is a summary of an Aeonia Eye Refresh study performed by an independent third-party testing facility. We believe the information provided here is correct but past performance is not necessarily indicative of future results.